Human Immunodeficiency Virus And Mycobacterium avium Complex Coinfection Of Monocytoid Cells Results In Reciprocal Enhancement Of Multiplication

Disseminated Mycobacterium avium complex (MAC) infection is an important opportunistic infection in AIDS patients. Because cells of macrophage lineage are targets for both human immunodeficiency virus (HIV) and MAC, the monocytoid cell line U937 was coinfected with both pathogens. Coinfected cultures had increased HIV replication (more than threefold at day 6) and an increased percentage of HIV-infected cells compared with cultures infected only with HIV. The kinetics of HIV replication were significantly increased in this coinfection system as measured by flow cytometry. When cells were infected concurrently, the rate of intracellular growth of MAC was not significantly affected. However, cells preinfected with HIV before infection with MAC showed significant enhancement of MAC growth compared with control cells. The kinetics of cell death were also increased in the coinfection system compared with singly infected controls. Thus, coinfection of monocytoid cells with HIV and MAC in vitro results in reciprocal enhancement of multiplication.