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February 10, 1976Biochemistry

Isolation of viral specific RNA from SV40 infected cells by viral DNA chemically linked to a cellulose matrix

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Population

SV40 infected cells

Design

Preclinical

Authors

TSThomas Y. ShihHokkaido UniversityGKGeorge KhouryLebanese American University

Discussion

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Implication

May aid viral RNA isolation in cell models; leaves open translation to human or clinical applications.

Structured PICO

P
Population
SV40 infected cells
I
Intervention
SV40 DNA fragments chemically attached to neutral cellulose powder with a water-soluble carbodiimide
O
Outcome
Isolation of late lytic viral specific RNAsurrogate

A method using SV40 DNA chemically linked to a cellulose matrix successfully isolates highly pure viral specific RNA from infected cells.

Cite This Study

Shih et al. (1976) studied this question.

synapsesocial.com/papers/6a945e488d39fd7482c7cde6https://doi.org/10.1021/bi00648a005
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