Key result
Quantitative in situ hybridization achieved +/-10% reproducibility in detecting SV40 replication in monkey kidney cells, but could not detect the integrated genome in transformed cells.
Population
SV40-monkey kidney cells (CV-1) and SV40-transformed cells
Design
Preclinical
Authors
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Quantitative in situ hybridization reliably detects lytic SV40 but misses integrated genomes; leaves open need for sensitive assays in transformation studies.
Quantitative in situ hybridization can reproducibly detect SV40 replication in lytic infection but lacks sensitivity for detecting integrated genomes in transformed cells.
Langelier et al. (1975) studied SV40 infection. In situ hybridization was evaluated on Detection of viral DNA synthesis. Quantitative in situ hybridization achieved +/-10% reproducibility in detecting SV40 replication in monkey kidney cells, but could not detect the integrated genome in transformed cells.
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