Key result
Permanent atrial fibrillation in patients with mitral valve disease was associated with differential expression of cytoskeleton, energetic metabolism, and cytoprotection proteins compared to sinus rhythm.
Cross-Sectional (n=17)
Proteomic analysis reveals differential expression of cytoskeleton, metabolic, and cytoprotective proteins in the atrial appendages of patients with mitral valve disease and AF compared to sinus rhythm, suggesting potential new drug targets.
Should not yet change practice in mitral valve disease; hypothesis-generating for proteomic targets in permanent AF.
INTRODUCTION: The objective was to compare by proteomics the expression of proteins associated with the cytoskeleton, energetic metabolism, and cardiac cytoprotection between left atrial appendages (LAA) and right atrial appendages (RAA) obtained from patients with mitral valve disease both in sinus rhythm (SR, n = 6) and in permanent atrial fibrillation (AF, n = 11). METHODS AND RESULTS: Samples from RAA and LAA were obtained from the same patient. Proteins were separated in 2-dimensional electrophoresis and identified by mass spectrometry. LAA from SR patients upexpressed alpha-actin isotype 1 and desmin isotypes 3 and 5 with respect to RAA. In LAA from AF patients were upexpressed cardiac alpha-actin isotypes 1 and 2, tropomyosin alpha- and beta-chains, and myosin light chain embryonic muscle/atrial isoform with respect to LAA from SR patients. In RAA from AF patients also upexpressed different cytoskeleton associated proteins with respect to RAA from SR patients. Different energetic metabolism-associated proteins were upexpressed in LAA and RAA from AF with respect those from SR patients. In AF patients, the expression of proteins associated with cardiac cytoprotection such as gluthatione-S-transferase, heat shock protein (Hsp) 27, and different Hsp60 isotypes, were higher in RAA but not in LAA with respect to the corresponding appendages in SR patients. CONCLUSIONS: For each individual patient RAA and LAA showed a similar level of proteins expressed associated with cytoskeleton, energetic metabolism, and cardiac cytoprotection. There were more differences in the level of proteins associated with the above-mentioned mechanisms between the atrial appendages from AF with respect to SR patients, which may open new targets for drugs.
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Modrego et al. (2010) conducted a cross-sectional in Mitral valve disease with sinus rhythm or permanent atrial fibrillation (n=17). Permanent atrial fibrillation vs. Sinus rhythm was evaluated on Expression of proteins associated with the cytoskeleton, energetic metabolism, and cardiac cytoprotection. Permanent atrial fibrillation in patients with mitral valve disease was associated with differential expression of cytoskeleton, energetic metabolism, and cytoprotection proteins compared to sinus rhythm.
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