The use of Sephadex G-50 and Chelex-100 resins removes PCR inhibitors from sludge-amended soil, enabling the detection of enteroviruses.
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Enables enterovirus monitoring in sludge-amended soils; leaves open validation and environmental risk implications.
PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.
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Straub et al. (1994) studied this question.
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