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May 23, 2006Journal of Agricultural and Food Chemistry

Adulteration of Apple with Pear Juice: Emphasis on Major Carbohydrates, Proline, and Arbutin

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Authors

PTPushparajah ThavarajahNLNicholas H. Low

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Overview

Analytical study reveals arbutin reliably identifies pear juice adulteration in apple juice, highlighting a robust detection method.

Key Points

  • To evaluate major carbohydrates, polyols, proline, and phenolic profiles to identify reliable chemical markers for detecting the adulteration of apple juice with pear juice.
  • Analyzed 105 authentic apple juice samples across 13 countries and 27 authentic pear juice samples across 5 countries.
  • Profiled major carbohydrates (fructose, glucose, sucrose), sorbitol, and proline levels across all juice samples.
  • Developed an HPLC-PDA method to quantify arbutin and implemented an enzymatic confirmation assay utilizing beta-glucosidase to hydrolyze arbutin to hydroquinone.
  • Major carbohydrate profiles significantly overlapped, and broad natural variations in sorbitol and proline permitted undetected pear adulteration at levels up to 30%.
  • Arbutin was present in all 27 authentic pear juice samples and absent (<0.5 µg/mL) in all 105 apple juice samples.
  • The HPLC-PDA analytical method detected pear juice adulteration in apple juice down to a limit of 2% (v/v).

Cite This Study

Thavarajah et al. (2006) studied this question.

synapsesocial.com/papers/6a94cfba9183a61d30168b37https://doi.org/10.1021/jf060259m
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