Key result
Three new ELISAs demonstrated differential recognition of intact and proteolyzed IGFBP-3 variants, with ELISA-1 measuring intact IGFBP-3, ELISA-3 measuring total, and ELISA-2 preferring fragments.
Population
Normal adult sera (n = 26), seminal plasma (n = 14), pregnancy sera (n = 30), and amniotic fluid (n = 30)
Comparison
Three new enzyme-linked immunosorbent assays for… vs Established method
Design
Preclinical
Authors
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Requires human validation before clinical use; leaves open IGFBP-3 proteolysis assessment in disease.
The development of three new ELISAs allows for the differential determination of intact and proteolyzed IGFBP-3 variants, which may help define their physiological and clinical importance.
Diamandi et al. (2000) studied IGFBP-3 proteolysis and measurement (n=100). Three new ELISAs (ELISA-1, -2, -3) for IGFBP-3 vs. Established method (Diagnostic Systems Laboratories, Inc., active IGFBP-3 ELISA) was evaluated on Detection of IGFBP-3 levels and variants. Three new ELISAs demonstrated differential recognition of intact and proteolyzed IGFBP-3 variants, with ELISA-1 measuring intact IGFBP-3, ELISA-3 measuring total, and ELISA-2 preferring fragments.