Key result
Depletion of H-specific antibodies resulted in an almost complete reduction of measles virus neutralization activity, whereas depletion of F-specific antibodies had minimal effect.
Why the study?
Does depletion of hemagglutinin- or fusion protein-specific antibodies reduce measles virus neutralization activity in sera from vaccinated young adults?
Does depletion of hemagglutinin- or fusion protein-specific antibodies reduce measles virus neutralization activity in sera from vaccinated young adults?
Measles virus neutralizing antibodies induced by vaccination are mainly directed against the hemagglutinin (H) protein rather than the fusion (F) protein.
H-specific antibodies drive post-vaccination measles neutralization; leaves open optimal antigen design for next-generation human vaccines.
The relative contribution of measles virus hemagglutinin (H)- or fusion protein (F)-specific antibodies to virus neutralization (VN) has not been demonstrated. We have depleted these specific antibodies from sera collected from young adults, who had been vaccinated during childhood, by prolonged incubation with viable transfected human melanoma cells expressing H or F. Simultaneous depletion of antibodies of both specificities completely abrogated VN activity. Depletion of F-specific antibodies only had a minimal effect, whereas removal of H-specific antibodies resulted in almost complete reduction of VN activity. These results demonstrate that measles virus neutralizing antibodies are mainly directed to the H protein.
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Swart et al. (2005) studied Healthy (measles vaccinated) (n=96). Depletion of H- or F-specific antibodies vs. No depletion (incubation with untransfected cells) was evaluated on Virus neutralization (VN) activity. Depletion of H-specific antibodies resulted in an almost complete reduction of measles virus neutralization activity, whereas depletion of F-specific antibodies had minimal effect.
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