What question did this study set out to answer?

To determine whether the internal ribosome entry site of Taura syndrome virus initiates translation using its in-frame AUG codon and canonical eukaryotic initiation factors.

December 21, 2004Journal of VirologyOpen Access

Factor-Independent Assembly of Elongation-Competent Ribosomes by an Internal Ribosome Entry Site Located in an RNA Virus That Infects Penaeid Shrimp

Q: What is the clinical evidence from this study?

Study design: Other. Population: Taura syndrome virus (TSV) infection. Intervention: Taura syndrome virus (TSV) intergenic region (IGR) internal ribosome entry site (IRES) vs. CrPV IRES, EMCV IRES, or no insert. Primary outcome: Factor-independent assembly of 80S ribosomes and translation initiation.

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Key result

The Taura syndrome virus (TSV) internal ribosome entry site (IRES) can assemble 80S ribosomes and direct protein synthesis in the absence of initiation factors and initiator tRNA.

Population

Taura syndrome virus internal ribosome entry site and reconstituted translation system

Design

Preclinical

Authors

RCRandal C. CevallosStanford University PSPeter SarnowStanford University

Discussion

Member takes

Implication

May inform invertebrate antiviral strategies; leaves open extension to mammalian translation or human pathogens.

Key Points

To determine whether the internal ribosome entry site of Taura syndrome virus initiates translation using its in-frame AUG codon and canonical eukaryotic initiation factors.
Analyzed translation initiation mechanisms mediated by the intergenic region internal ribosome entry site of Taura syndrome virus.
Reconstituted translation in vitro using purified ribosomal subunits, eukaryotic elongation factors 1A and 2, and aminoacylated tRNAs in the absence of initiation factors.
Demonstrated that the viral internal ribosome entry site does not require its in-frame AUG codon or any other AUG codon to initiate protein synthesis.
Assembled functional 80S ribosomes and synthesized proteins using solely purified ribosomal subunits, elongation factors, and aminoacylated tRNAs without initiation factors.

Structured PICO

Population

Taura syndrome virus (TSV) internal ribosome entry site (IRES) and reconstituted translation system (purified ribosomal subunits, eukaryotic elongation factors 1A and 2, and aminoacylated tRNAs)

Exposure

Translation initiation by TSV IRES

Outcome

Assembly of 80S ribosomes and protein synthesis in the absence of initiation factors

The TSV IRES initiates translation at non-AUG codons by an initiation factor-independent mechanism, similar to the cricket paralysis virus IRES.

Limitations

Structural analyses on the TSV IRES have not yet been reported

Cite This Study

Cevallos et al. (2004) studied Taura syndrome virus (TSV) infection. Taura syndrome virus (TSV) intergenic region (IGR) internal ribosome entry site (IRES) vs. CrPV IRES, EMCV IRES, or no insert was evaluated on Factor-independent assembly of 80S ribosomes and translation initiation. The Taura syndrome virus (TSV) internal ribosome entry site (IRES) can assemble 80S ribosomes and direct protein synthesis in the absence of initiation factors and initiator tRNA.

synapsesocial.com/papers/6a95062195b4276a8af577de https://doi.org/10.1128/jvi.79.2.677-683.2005

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Factor-Independent Assembly of Elongation-Competent Ribosomes by an Internal Ribosome Entry Site Located in an RNA Virus That Infects Penaeid Shrimp

Discussion

Member takes

Implication

Key Points

Structured PICO

Limitations

Cite This Study

Factor-Independent Assembly of Elongation-Competent Ribosomes by an Internal Ribosome Entry Site Located in an RNA Virus That Infects Penaeid Shrimp

Discussion

Member takes

Implication

Key Points

Structured PICO

Limitations

Cite This Study

Also Consider

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