February 1, 1999Journal of VirologyOpen Access

Translation Initiation at the CUU Codon Is Mediated by the Internal Ribosome Entry Site of an Insect Picorna-Like Virus In Vitro

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Population

In vitro model using dicistronic and monocistronic RNAs of Plautia stali intestine virus (PSIV)

Design

Preclinical

Authors

Discussion

Challenges canonical codon recognition; leaves open relevance to mammalian translation or antiviral strategies.

Population

In vitro model using dicistronic and monocistronic RNAs of Plautia stali intestine virus (PSIV)

Intervention

Deletion analysis and site-directed mutagenesis of the internal ribosome entry site (IRES) and initiation codon

Outcome

Translation mechanism and initiation codon of the capsid protein gene

Demonstrates that the PSIV IRES can effectively direct translation initiation at a non-AUG (CUU) codon without stable codon-anticodon pairing.

Sasaki et al. (1999) studied this question.

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