Key result
IL-17 protein and mRNA levels in PHA-stimulated PBMCs were significantly elevated in patients with dilated cardiomyopathy compared to normal controls and strongly correlated with heart function.
Why the study?
Are IL-17 levels in peripheral blood mononuclear cells elevated in patients with dilated cardiomyopathy and correlated with disease severity?
Case-Control (n=50)
Are IL-17 levels in peripheral blood mononuclear cells elevated in patients with dilated cardiomyopathy and correlated with disease severity?
IL-17 expression in PBMCs is elevated in patients with dilated cardiomyopathy and correlates with heart function, suggesting a potential role of immune activation in DCM pathogenesis.
IL-17 elevation in PBMCs associates with DCM severity; leaves open causal role and therapeutic targeting pending prospective studies.
OBJECTIVE: The objective of the study was to investigate the relation between interleukin-17 (IL-17) level in the plasma and in peripheral blood mononuclear cells (PBMCs), and dilated cardiomyopathy (DCM) disease severity. METHODS AND RESULTS: 30 patients with DCM and 20 normal adults as control were studied. IL-17 protein level in plasma, PBMC culture supernatants, and phytohaemagglutinin-stimulated PBMC culture supernatants were measured with ELISA. IL-17 mRNA expression in PBMCs was detected by reverse transcription-polymerase chain reaction. Results showed that the IL-17 protein level in PHA-stimulated PBMC culture supernatants or its mRNA level in the PHA-stimulated PBMC, but not in plasma or in PBMC culture supernatants, was significantly elevated in DCM patients compared with normal control subjects.The IL-17 protein level in cultured supernatants and the IL-17 mRNA level in the PBMCs were strongly correlated with the heart function of DCM patients. CONCLUSIONS: The ability to express IL-17 protein or mRNA in PBMC is abnormal and the change strongly correlates with the heart function of DCM patients.
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Yanlan Huang (2009) conducted a case-control in Dilated cardiomyopathy (n=50). Dilated cardiomyopathy vs. Normal adults was evaluated on IL-17 protein and mRNA levels in PHA-stimulated PBMCs. IL-17 protein and mRNA levels in PHA-stimulated PBMCs were significantly elevated in patients with dilated cardiomyopathy compared to normal controls and strongly correlated with heart function.
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