Key result
Manipulation of SKCa channels via overexpression or dominant-negative suppression profoundly altered the medium AHP current but had no effect on the slow AHP current, indicating that SKCa channels do not mediate the slow AHP in cortical pyramidal neurons.
Population
Pyramidal cells of layer V in cultured brain slices and a transgenic mouse expressing a truncated SK subunit
Design
Preclinical
Authors
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Challenges SKCa mediation of slow AHP; leaves open distinct molecular mediators for future validation in cortical neurons.
SK(Ca) channels mediate the medium but not the slow calcium-activated afterhyperpolarization in cortical pyramidal neurons.
Villalobos et al. (2004) studied None (Basic science study on cortical neurons). SKCa channel manipulation (overexpression of KCa2.1/KCa2.2 or dominant-negative SK3-1B) vs. Untransfected cells or wild-type mice was evaluated on Amplitude of slow AHP current (IsAHP) and medium AHP current (ImAHP). Manipulation of SKCa channels via overexpression or dominant-negative suppression profoundly altered the medium AHP current but had no effect on the slow AHP current, indicating that SKCa channels do not mediate the slow AHP in cortical pyramidal neurons.
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