June 8, 1999Proceedings of the National Academy of SciencesOpen Access

Regulation of eukaryotic protein synthesis: Selective influenza viral mRNA translation is mediated by the cellular RNA-binding protein GRSF-1

View Full Paper

Ask AI

Bookmark

Population

Yeast strains (L40-coat, R40-coat), HeLa cell extracts, and E. coli for recombinant protein expression

Comparison

Cellular RNA-binding protein GRSF-1 vs Mutant NP 5' UTRs, cellular RNA 5' UTRs, and…

Design

Preclinical

Authors

YPYoung Woo ParkUnifor JWJeffrey WiluszColorado State University MKMichael G. KatzeUniversity of Washington

Discussion

Member takes

Implication

GRSF-1 may mediate influenza mRNA translation; leaves open in vivo targeting as antiviral strategy.

Structured PICO

Population

Yeast strains (L40-coat, R40-coat), HeLa cell extracts, and E. coli for recombinant protein expression

Intervention

Cellular RNA-binding protein GRSF-1

Comparator

Mutant NP 5' UTRs, cellular RNA 5' UTRs (SEAP), and other RNA-binding proteins (PTB, U1A)

Outcome

Binding affinity to influenza virus NP 5' UTR and translation efficiency of NP 5' UTR-driven templates

The cellular RNA-binding protein GRSF-1 selectively binds to the 5' UTR of influenza viral mRNAs to mediate their preferential translation in infected cells.

Cite This Study

Park et al. (1999) studied this question.

synapsesocial.com/papers/6a954afe6a42edee3f2ca696 https://doi.org/10.1073/pnas.96.12.6694

View Full Paper

Ask AI

Bookmark

Regulation of eukaryotic protein synthesis: Selective influenza viral mRNA translation is mediated by the cellular RNA-binding protein GRSF-1

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Regulation of eukaryotic protein synthesis: Selective influenza viral mRNA translation is mediated by the cellular RNA-binding protein GRSF-1

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider