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Objective: To develop UV spectrophotometric assay validation methods for some selected medicinal plant extracts. Methods : Dried, powdered leaves of Annona muricata (AM) and Andrographis paniculata (AP) as well as seeds of Garcinia kola (GK) and Hunteria umbellata (HU) were separately subjected to maceration using distilled water. Different concentrations of the extracts were scanned spectrophotometrically to obtain wavelengths of maximum absorbance. The different extracts were then subjected to validation studies following international guidelines at the respective wavelengths obtained. Results: The results showed linearity at peak wavelengths of maximum absorbance of 292, 280, 274 and 230 nm for GK, HU, AM and AP, respectively. The calibration curves for the different concentrations of the extract gave R2 values ranging from 0.9831 for AM to 0.9996 for AP the inter-day and intra-day precision study showed that the relative standard deviation (%) was ? 10% for all the extracts. Conclusion : The aqueous extracts and isolates of these plants can be assayed and monitored using these wavelengths.
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Arhewoh et al. (2014) studied this question.
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