Metabolism of 2,4,4'-trichlorobiphenyl by Acinetobacter sp. P6.

Metabolism of 2, 4, 4'-trichlorobiphenyl by Acinetobacter sp. strain P6 has been studied. When the incubation was carried out without shaking at 15°C, two isomeric monohydroxy compounds, a dihydrodiol compound, a dihydroxy compound, a meta-cleaved yellow com-pound and a dichlorobenzoic acid were detected by combined gas liquid chromatograph-mass spectrometry. As an additional metabolite, dichlorodihydroxy biphenyl, a dechlorination-hydroxylation product, was also detected. When the incubation mixture was shaken at 30°C, a meta-cleaved yellow compound was readily produced and predominantly accumulated in the reaction mixture upon further incubation. The major pathway of 2, 4, 4'-trichloro-biphenyl by Acinetobacter sp. P6 was considered to proceed oxidatively via 2, ' 3'-dihydro-2', 3'-diol compound, concomitant dehydrogenated 2', 3'-dihydroxy compound and then the 1', 2'-meta-cleaved yellow compound, i.e., 3-chloro-2-hydroxy-6-oxo-6 (2, 4-dichlorophenyl) hexa-2, 4-dienoic acid.