What question did this study set out to answer?

To determine how autologous alveolar and peritoneal macrophages affect DNA synthesis in resting and antigen-stimulated spleen cell cultures.

November 1, 1966The Journal of Immunology

Inhibition of Spleen Cell DNA Synthesis by Autologous Macrophages

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Authors

RPR. M. E. ParkhouseInstituto Gulbenkian de Ciência RDRichard DuttonElon University

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Implication

In vitro experiment demonstrates contact-dependent inhibition of spleen cell DNA synthesis by autologous macrophages, indicating a regulatory mechanism limiting lymphocyte proliferation.

Key Points

To determine how autologous alveolar and peritoneal macrophages affect DNA synthesis in resting and antigen-stimulated spleen cell cultures.
Measured DNA synthesis in vitro across spleen cell suspensions co-cultured with autologous alveolar macrophages, peritoneal macrophages, or polymorphs.
Tested the role of soluble factors, serum, cell homogenization, nutrient depletion, and intimate cell-to-cell contact on macrophage-mediated inhibition.
Assessed the effect of eliminating resident macrophages in spleen suspensions using silica treatment.
Addition of intact autologous alveolar or peritoneal macrophages suppressed DNA synthesis in control and antigen-stimulated spleen cells only when intimate cell contact occurred.
Inhibition was independent of serum, was not mediated by soluble factors or nutrient depletion, and did not occur when using cell homogenates or autologous polymorphs.
Depletion of endogenous spleen macrophages by silica treatment resulted in a small stimulation of DNA synthesis.

Cite This Study

Parkhouse et al. (1966) studied this question.

synapsesocial.com/papers/6a9596c3c449400cd78292ea https://doi.org/10.4049/jimmunol.97.5.663

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Implication

In vitro experiment demonstrates contact-dependent inhibition of spleen cell DNA synthesis by autologous macrophages, indicating a regulatory mechanism limiting lymphocyte proliferation.

Inhibition of Spleen Cell DNA Synthesis by Autologous Macrophages

Discussion

Member takes

Implication

Key Points

Cite This Study

Inhibition of Spleen Cell DNA Synthesis by Autologous Macrophages

Discussion

Member takes

Implication

Key Points

Cite This Study

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