Modified Bouin's solution or enzyme-treated formalin-fixed tissues can be effectively used for routine diagnostic detection of IBDV and IBV in chickens.
Immunoperoxidase and immunofluorescent techniques were used to detect and localise infectious bursal disease virus (IBDV) and infectious bronchitis virus (IBV) in fixed, paraffin-embedded chicken tissues. A modified Bouin's solution (9 parts of 1% picric acid to 1 part of 10% neutral buffered formalin and 5% acetic acid) proved to be the fixative of choice. Tissues fixed in 10% neutral buffered formalin showed heavy background staining, whereas enzyme treatment of these tissues succeeded in exposing viral antigens and thus giving a specific reaction. For routine diagnostic purposes it will prove advantageous to detect IBDV and IBV in fixed, paraffin-embedded material.
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Jönsson et al. (1986) studied this question.
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