Polyacrylamide disc electrophoresis developed by Davis (4) was investigated to determine its effectiveness in resolving whey proteins and for assessing the effect of heat on milk proteins. This technique was chosen because of its high resolution properties with protein solutions. The major whey protein hands are identified as fi-lactoglobulin A and B, a-lactalbumin, bovine serum albumin (BSA), and the immune globulins in decreasing order of mobility.
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Hartman et al. (1965) studied this question.
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