Key result
Application of SHAPE-MaP to the porcine epidemic diarrhea virus RNA genome identified functional regions and secondary structures that can be exploited by siRNAs to inhibit viral proliferation.
Why the study?
Designing RNA-targeting compounds or siRNAs to control viral infections requires identifying conserved and functional viral RNA structures in cells.
SHAPE-MaP is an effective method to identify secondary structures in viral RNA genomes that can be targeted to inhibit viral proliferation.
RNA-targeting compounds or small interfering RNAs (siRNAs) offer a potent means to control viral infections. An essential prerequisite for their design depends on identifying conserved and functional viral RNA structures in cells. Techniques that probe RNA structures in situ have recently been developed including SHAPE-MaP, which has been helpful in the analysis of secondary structures of RNA. In this study, we report on the application of SHAPE-MaP to the porcine epidemic diarrhea virus RNA genome to categorize different functional regions, including potential quadruplex-forming sequence and target sites of siRNA. Our results show that these structures can be exploited to inhibit viral proliferation and that SHAPE-MaP is an effective method to identify secondary structures in RNA genomes.
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Luo et al. (2025) studied Porcine epidemic diarrhea virus infection. SHAPE-MaP and RNA-targeting compounds/siRNAs was evaluated on Identification of secondary structures and viral proliferation. Application of SHAPE-MaP to the porcine epidemic diarrhea virus RNA genome identified functional regions and secondary structures that can be exploited by siRNAs to inhibit viral proliferation.
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