Key result
Targeting the dynamic single-stranded regions of the PEDV RNA genome with the G-quadruplex stabilizer Braco-19 (EC50 3.60 µM) or specific siRNAs significantly inhibited viral proliferation in vitro.
Why the study?
Designing RNA-targeting compounds or siRNAs to control viral infections requires identifying conserved and functional viral RNA structures in cells.
Effect estimate: EC50 3.60 µM
SHAPE-MaP is an effective method for identifying secondary structures in viral RNA genomes to design targeted RNA-targeting compounds or siRNAs.
RNA-targeting compounds or small interfering RNAs (siRNAs) offer a potent means for controlling viral infections. An essential prerequisite to their design depends on identifying conserved and functional viral RNA structures in cells. Techniques that probe RNA structures in situ have been developed recently including SHAPE-MaP, which has been helpful in analyzing the secondary structures of RNA. In this study, we report on the application of SHAPE-MaP to the Porcine Epidemic Diarrhoea Virus (PEDV) RNA genome to categorize different functional regions including potential quadruplex forming sequence and target sites of small interfering RNA (siRNA). Our results show that these structures can be exploited to inhibit viral proliferation and that SHAPE-MaP is an effective method to the identification of secondary structures in RNA genomes.
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Luo et al. (2024) studied Porcine Epidemic Diarrhoea Virus (PEDV) infection. Braco-19 and targeted siRNAs vs. Untreated or non-targeting siRNA control was evaluated on Viral proliferation and RNA synthesis inhibition (EC50 3.60 µM). Targeting the dynamic single-stranded regions of the PEDV RNA genome with the G-quadruplex stabilizer Braco-19 (EC50 3.60 µM) or specific siRNAs significantly inhibited viral proliferation in vitro.
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