What is the clinical evidence from this study?

Study design: Other. Population: Hepatitis C virus (HCV). Intervention: siRNA-induced silencing of hnRNP K. Primary outcome: HCV replication efficiency.

October 11, 2013Molecular & Cellular ProteomicsOpen Access

A Human Proteome Microarray Identifies that the Heterogeneous Nuclear Ribonucleoprotein K (hnRNP K) Recognizes the 5′ Terminal Sequence of the Hepatitis C Virus RNA

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Key result

Knockdown of heterogeneous nuclear ribonucleoprotein K (hnRNP K) reduced HCV replication in a concentration-dependent manner, identifying it as a key cellular protein binding to HCV SL1.

Population

Human proteome microarray and Huh7.5 cells stably expressing HCV replicon

Design

Preclinical

Authors

BFBaochang FanNational Central University KLKuan‐Yi LuUniversity of North Carolina at Chapel Hill FSF.X. Reymond SutandyNational Central University

Discussion

Member takes

Implication

Hypothesis-generating in animal models; leaves open hnRNP K as a human HCV target pending clinical validation.

Structured PICO

Population

Human proteome microarray (∼17,000 individually purified human proteins in full-length) and Huh7.5 cells stably expressing HCV replicon

Intervention

siRNA-induced silencing of candidate genes (specifically heterogeneous nuclear ribonucleoprotein K [hnRNP K])

Outcome

HCV replication efficiency and binding to HCV SL1surrogate

Identifies hnRNP K as a cellular protein that binds to HCV SL1 and regulates HCV replication.

Cite This Study

Fan et al. (2013) studied Hepatitis C virus (HCV). siRNA-induced silencing of hnRNP K was evaluated on HCV replication efficiency. Knockdown of heterogeneous nuclear ribonucleoprotein K (hnRNP K) reduced HCV replication in a concentration-dependent manner, identifying it as a key cellular protein binding to HCV SL1.

synapsesocial.com/papers/6a9644c0ff8a6b2115f6962d https://doi.org/10.1074/mcp.m113.031682

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October 11, 2013Molecular & Cellular ProteomicsOpen Access

A Human Proteome Microarray Identifies that the Heterogeneous Nuclear Ribonucleoprotein K (hnRNP K) Recognizes the 5′ Terminal Sequence of the Hepatitis C Virus RNA

View Full Paper

Ask AI

Bookmark

Key result

Knockdown of heterogeneous nuclear ribonucleoprotein K (hnRNP K) reduced HCV replication in a concentration-dependent manner, identifying it as a key cellular protein binding to HCV SL1.

Population

Human proteome microarray and Huh7.5 cells stably expressing HCV replicon

Design

Preclinical

Authors

BFBaochang FanNational Central University KLKuan‐Yi LuUniversity of North Carolina at Chapel Hill FSF.X. Reymond SutandyNational Central University

Discussion

Member takes

Implication

Hypothesis-generating in animal models; leaves open hnRNP K as a human HCV target pending clinical validation.

Structured PICO

Population

Human proteome microarray (∼17,000 individually purified human proteins in full-length) and Huh7.5 cells stably expressing HCV replicon

Intervention

siRNA-induced silencing of candidate genes (specifically heterogeneous nuclear ribonucleoprotein K [hnRNP K])

Outcome

HCV replication efficiency and binding to HCV SL1surrogate

Identifies hnRNP K as a cellular protein that binds to HCV SL1 and regulates HCV replication.

Cite This Study

synapsesocial.com/papers/6a9644c0ff8a6b2115f6962d https://doi.org/10.1074/mcp.m113.031682

Also Consider

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