Key result
Inoculation with porcine encephalomyocarditis virus strain BD2 resulted in 100% mortality in BALB/c mice within 7 days, with the virus replicating mainly in brain tissues and inducing acute encephalitis.
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Porcine EMCV strain BD2 isolated from China is highly virulent in BALB/c mice, causing 100% mortality and acute encephalitis, establishing a viable experimental animal model.
Encephalomyocarditis virus (EMCV) can cause acute myocarditis in young pigs or reproductive failure in sows. The BD2 strain was isolated from the suspected piglets with EMCV in China. In order to establish an experimental animal model of EMCV, eight-weeks-old male BALB/c mice were intraperitonealy inoculated with 0.1 ml of 4×10(5) TCID50 suspension of the EMCV. Infected mice demonstrated hind limb paralysis, and movement disorder. The mortality rate of the infected group was 100% during the one-week observation period. The viral load in the brain of challenged mice gradually increased, with a peak level being 6.53 log CCID50/0.1 ml 5 days post infection. The pathological injury in infected mice was presented as neuronal necrosis. Brown positive staining could be detected in the cytoplasm of cerebral neurons. These results indicate that the porcine EMCV isolated from China could replicate in brain tissues and induce acute encephalitis in BALB/c mice.
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Yuan et al. (2015) studied Encephalomyocarditis virus (EMCV) infection (n=50). Porcine encephalomyocarditis virus strain BD2 vs. Mock inoculation with BHK-21 cell culture supernatant was evaluated on Mortality rate (p=<0.001). Inoculation with porcine encephalomyocarditis virus strain BD2 resulted in 100% mortality in BALB/c mice within 7 days, with the virus replicating mainly in brain tissues and inducing acute encephalitis.
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