Key result
In experimental diabetic rats, L-type Ca2+ channel current densities in vascular smooth muscle cells were reduced and showed increased sensitivity to cAMP (P < 0.01) and hyperglycemia.
p-value: p=<0.01
Diabetic vascular smooth muscle cells exhibit altered L-type calcium channel properties, including reduced current density and increased sensitivity to cAMP and hyperglycemia, which may underlie diabetic vascular complications.
May underlie diabetic vascular complications; hypothesis-generating and requires validation in human models before clinical consideration.
Vascular complications of diabetes are associated with abnormal Ca(2+) handling by vascular smooth muscle cells (SMCs) in which the alteration in L-type voltage-dependent Ca(2+) channel (VDCC) currents may play an important role. In the present study, the characteristics of L-type VDCC currents in tail artery SMCs from streptozotocin-induced diabetic rats were examined. The densities, but not the voltage dependence, of L-type VDCC currents were reduced as diabetes progressed from 1 wk to 3 mo. The inhibitory effect of dibutyryl-cAMP on L-type VDCC currents was greater in diabetic SMCs than in age-matched control cells (P < 0.01). Both the stimulatory effect of BAY K 8644 and the inhibitory effect of nifedipine on L-type VDCC currents were significantly enhanced in diabetic cells. The diabetes-related abnormalities in L-type VDCC currents were mimicked by culturing SMCs with a high concentration of glucose. Our results suggest that the properties of L-type VDCC in diabetic vascular SMCs were significantly altered, partially related to the increased L-type VDCC sensitivity to cAMP and hyperglycemia.
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Wang et al. (2000) studied Diabetes (experimental). Streptozotocin-induced diabetes / High glucose exposure vs. Age-matched control cells was evaluated on L-type voltage-dependent Ca(2+) channel (VDCC) currents characteristics (p=<0.01). In experimental diabetic rats, L-type Ca2+ channel current densities in vascular smooth muscle cells were reduced and showed increased sensitivity to cAMP (P < 0.01) and hyperglycemia.
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