Key result
Steroidal saponins extract from Ophiopogon japonicus root (100 mg/kg) improved ejection fraction to 68.26% and reduced inflammatory and oxidative stress markers in a rat model of doxorubicin-induced CHF.
Why the study?
Ophiopogonis Radix is investigated for treating cardiovascular diseases, but the cardioprotective effects of steroidal saponins extract from Ophiopogon japonicus root against doxorubicin-induced chronic heart failure required evaluation.
Does steroidal saponins extract from Ophiopogon japonicus root improve echocardiographic and biochemical parameters in a rat model of doxorubicin-induced chronic heart failure?
RCT (n=32)
randomly divided
Does steroidal saponins extract from Ophiopogon japonicus root improve echocardiographic and biochemical parameters in a rat model of doxorubicin-induced chronic heart failure?
Steroidal saponins extract from Ophiopogon japonicus root demonstrates cardioprotective effects in a rat model of doxorubicin-induced heart failure by reducing oxidative stress and inflammation.
Should not yet change heart failure practice; leaves open translation of saponin cardioprotection from rat models to patients.
CONTEXT: Ophiopogonis Radix, the root of Ophiopogon japonicus (Thunb.) Ker-Gawl (Liliaceae), is a Traditional Chinese Medicine, which has been investigated to possess effective treatment of cardiovascular diseases. OBJECTIVE: This study evaluates the cardioprotective effects of steroidal saponins extract from Ophiopogon japonicus (SOJ) root against doxorubicin-induced chronic heart failure (CHF) through the amelioration of oxidative stress and inflammation. MATERIALS AND METHODS: A Sprague-Dawley rat model of CHF was established by intraperitoneally injected with DOX. All rats were randomly divided into four groups: Control group, CHF group, CHF + SOJ (100 mg/kg) treatment group, SOJ (100 mg/kg) treatment group (n = 8/group). After six weeks administration, biometric and echocardiography were measured. The levels of biochemical parameters were measured using commercial kits. RESULTS: The values of LVESP, +dP/dtmax, -dP/dtmax, EF and FS increased to 116.20 ± 1.68 mmHg, 2978.71 ± 168.26 mmHg/s, 3452.61 ± 286.09 mmHg/s, 68.26 ± 5.28% and 31.97 ± 3.79%, respectively; the values of LVEDP, LVESD and LVEDD decreased to 8.85 ± 0.84 mmHg, 8.39 ± 0.45 mm and 12.36 ± 0.87 mm in CHF + SOJ group. In addition, the levels of IL-6, TNF-α and IL-1β decreased to 154.41 ± 7.72 pg/mg protein, 110.02 ± 6.96 pg/mg protein and 39.39 ± 5.27 pg/mg protein, respectively; the relative activity of p38 MAPK decreased to 2.60 ± 0.40 in CHF + SOJ group. Furthermore, the activities of SOD, CAT and GSH-Px increased to 268.77 ± 6.20 U/mg protein, 13.68 ± 0.68 U/mg protein and 316.90 ± 8.08 µmol/mg protein, and the content of MDA decreased to 4.03 ± 0.43 nmol/mg protein in CHF + SOJ group. CONCLUSIONS: SOJ exerts the cardioprotective effect against DOX-induced CHF through suppressing inflammatory and oxidative stress. These results provide evidence that SOJ might be an effective treatment for CHF.
No takes yet. Share an insight, caveat, or question.
Wu et al. (2019) conducted an RCT in doxorubicin-induced chronic heart failure (n=32). steroidal saponins extract from Ophiopogon japonicus (SOJ) root vs. Control group, CHF group was evaluated on echocardiographic and biochemical parameters. Steroidal saponins extract from Ophiopogon japonicus root (100 mg/kg) improved ejection fraction to 68.26% and reduced inflammatory and oxidative stress markers in a rat model of doxorubicin-induced CHF.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: