Key result
Differentiation of human neuroblastoma SK-N-SH subclones with 1mM dibutyryl cAMP resulted in higher resting membrane potentials (-50 to -80 mV) and the generation of action potentials.
Population
Human neuroblastoma cell line SK-N-SH subclones (SK-N-SH-IN and SK-N-SH-Sy5Y)
Comparison
Morphological differentiation induced by growth… vs Undifferentiated SK-N-SH parental cell line…
Design
Preclinical
Follow-up
1 week in culture
Authors
Loading...
Differentiated SK-N-SH subclones may refine neuronal models; leaves open any relevance to cardiovascular electrophysiology or clinical practice.
Morphological differentiation of human neuroblastoma cells induces the development of mature electrophysiological membrane properties, including the ability to generate action potentials.
Kuramoto et al. (1981) studied Human neuroblastoma. Dibutyryl cAMP vs. Undifferentiated SK-N-SH parental cell line was evaluated on Membrane properties (resting membrane potential, input resistance, time constants, spike potentials). Differentiation of human neuroblastoma SK-N-SH subclones with 1mM dibutyryl cAMP resulted in higher resting membrane potentials (-50 to -80 mV) and the generation of action potentials.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: