Key result
Thiol inhibitors at low concentrations inhibit the acto-H-meromyosin-forming ability of F-actin at low ionic strength, distinguishing enzymic active centers from interaction centers.
Population
F-actin and heavy meromyosin (in vitro biochemical model)
Design
Preclinical
Authors
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Distinguishes enzymatic from interaction sites in vitro; leaves open relevance to cardiac acto-myosin regulation.
This basic science study demonstrates that thiol reagents inhibit the interaction between F-actin and heavy meromyosin at low ionic strength, distinguishing the active centers for enzymatic activity from those for complex formation.
Perry et al. (1964) studied this question. Thiol inhibitors was evaluated on acto-H-meromyosin-forming ability of F-actin and enzymic activity. Thiol inhibitors at low concentrations inhibit the acto-H-meromyosin-forming ability of F-actin at low ionic strength, distinguishing enzymic active centers from interaction centers.
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