Key result
In rabbit muscle G-actin, Cys-257 was the most reactive thiol group to DACM, while upon polymerization to F-actin, practically all thiol groups became inaccessible.
Population
Actin from rabbit muscles
Design
Preclinical
Authors
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Actin polymerization may alter thiol accessibility in muscle; leaves open relevance to human cardiac actin dynamics.
The study demonstrates that Cys-257 is the most surface-exposed thiol in G-actin, and that polymerization to F-actin induces a drastic conformational change rendering all thiols inaccessible to DACM.
Liu et al. (1990) studied this question. 7-dimethylamino-4-methyl-(N-maleimidyl)coumarin (DACM) was evaluated on Accessibility of cysteine residues to DACM. In rabbit muscle G-actin, Cys-257 was the most reactive thiol group to DACM, while upon polymerization to F-actin, practically all thiol groups became inaccessible.
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