Key result
Fluorination of G-actin revealed that Cys-10 and -284 are not involved in polymerization, whereas the mobility of the 19F label at Cys-374 is markedly reduced upon polymerization.
Population
G-actin (globular protein, Mr 42 300)
Comparison
Reaction with 3-bromo-1,1,1-trifluoropropanone… vs Unmodified G-actin
Design
Preclinical
Authors
Loading...
Cys-374 mobility reduction may mark actin assembly; leaves open validation in cardiac cytoskeletal models.
The study demonstrates that the mobility of the Cys-374 residue in G-actin is markedly reduced upon polymerization to F-actin, while Cys-10 and -284 are unaffected.
Brauer et al. (1986) studied this question. 3-bromo-1,1,1-trifluoropropanone modification vs. Unmodified G-actin was evaluated on 19F NMR resonances and polymerization capability. Fluorination of G-actin revealed that Cys-10 and -284 are not involved in polymerization, whereas the mobility of the 19F label at Cys-374 is markedly reduced upon polymerization.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: