Key result
Most of the decrease in IAF fluorescence caused by the binding of MgATP to labeled myosin subfragment 1 is associated with the ATP-hydrolysis step.
Population
Myosin subfragment 1 (S-1) labeled with 5-(iodoacetamido)fluorescein (IAF) on the alkali 1 light chain
Design
Preclinical
Authors
Loading...
Offers mechanistic insight into myosin ATPase; leaves open relevance to human cardiac contractility or therapies.
The study demonstrates that the ATP-hydrolysis step causes a conformational change in the myosin S-1 molecule, altering the microenvironment of the bound IAF fluorophore.
Marsh et al. (1982) studied this question. MgATP was evaluated on Pre-steady-state kinetics of the fluorescence decrease of the IAF label. Most of the decrease in IAF fluorescence caused by the binding of MgATP to labeled myosin subfragment 1 is associated with the ATP-hydrolysis step.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: