Key result
In spontaneously hypertensive rats, cardiac hypertrophy development was associated with a prominent increase in the proportion of type V collagen compared to age-matched controls (p<0.01).
Hypothesis-generating in hypertensive rat models; leaves open type V collagen's role in human cardiac remodeling.
To determine how collagen is remodeled in the heart during the development of cardiac hypertrophy, the collagen concentration and the proportions of types I, III, and V collagen were analyzed in the hearts of 10-, 20-, 32-, and 40-week-old spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats. The collagen concentration was calculated after the hydroxyproline content was measured and the proportions of types I, III, and V collagen were determined by non-interrupted SDS-polyacrylamide gel electrophoresis (SDS-PAGE). There was no significant difference between the collagen concentrations in 10-week-old SHR and WKY. At 20 weeks, the collagen concentration in the hearts of the SHR had decreased significantly (p< 0.01) when compared to that in the WKY, at 40 weeks, this concentration in SHR had increased significantly (p<0.05) when compared to that in the WKY. Type V collagen in WKY increased with age and type I collagen in the 20- and 40-week-old WKY de-creased significantly (p<0.05) when compared to that in the 10-week-old WKY. However, the proportion of type I collagen in the 20-week-old WKY did not differ from that of the 40-week-old WKY. There was no significant difference between the proportions of the various types of collagen in 10-week-old SHR and those in age- and sex-matched WKY. However, the proportion of type V collagen in the 20- and 40-week-old SHR was significantly (p<0.01) higher than that in age- and sex-matched WKY. Moreover, the proportion of type I collagen in the 40-week-old SHR was significantly (p<0.01) lower than that in age- and sex-matched WKY. The III/I ratio of the 40-week-old SHR was significantly higher than that of age-matched WKY (p< 0.05). These results show qualitatively and quantitatively that collagen is remodeled as cardiac hypertrophy develops and that there is a prominent increase in type V collagen. This suggests that type V collagen is involved in the pathogenesis of cardiac hypertrophy in SHR.
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Honda et al. (1993) studied Cardiac hypertrophy. Spontaneous hypertension vs. Wistar-Kyoto (WKY) rats was evaluated on Collagen concentration and proportions of types I, III, and V collagen (p=<0.01). In spontaneously hypertensive rats, cardiac hypertrophy development was associated with a prominent increase in the proportion of type V collagen compared to age-matched controls (p<0.01).
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