Thin-layer chromatographic separation of sulfated and nonsulfated lithocholic acids and their glycine and taurine conjugates

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A method for superior thin-layer chromatographic separation of lithocholic acid and its N-glycine and N-taurine conjugates, as well as their respective 3alpha-sulfates, is described. A solvent system of chloroform-methanol-acetic acid-water 65:24:15:9 (v/v) is used with air-dried plates of silicic acid containing calcium sulfate (10% by weight) under conditions of chamber saturation.