Key result
A novel one-step hot start multiplex RT-PCR assay demonstrated high sensitivity and specificity for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples.
Population
150 positive field samples from several African swine fever and Classical swine fever outbreaks, whole blood…
Design
Preclinical
Authors
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May support multiplex RT-PCR development for swine fever diagnostics; leaves open routine adoption pending prospective validation.
A novel multiplex RT-PCR assay provides a rapid, sensitive, and specific tool for the simultaneous differential diagnosis of African and Classical swine fever.
Ag�ero et al. (2004) studied African swine fever and Classical swine fever (n=150). Multiplex RT-PCR assay vs. Uniplex PCR/RT-PCR was evaluated on Sensitivity and specificity for simultaneous detection of ASFV and CSFV. A novel one-step hot start multiplex RT-PCR assay demonstrated high sensitivity and specificity for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples.
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