Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
May 22, 2022Arabian Journal of ChemistryOpen Access

Integration of in silico and in vitro approaches to evaluate antioxidant and anticancer properties of Tribulus terrestris extracts

View Full Paper
Ask AI
Bookmark
Share

Authors

MSMuhammad Hussnain SiddiqueRARahat AndleebAAAsma Ashraf

Discussion

Loading...

Member takes

Overview

In vitro study reveals antioxidant, thrombolytic, and low cytotoxic activities of Tribulus terrestris seed extracts in HepG2 cells, suggesting potential for herbal drug standardization.

Key Points

  • To evaluate the phytochemical constituents, antioxidant efficacy, thrombolytic capacity, and anticancer properties of Tribulus terrestris seed extracts using experimental and computational approaches.
  • Quantified total phenolic (TPC) and flavonoid content (TFC) across methanolic, acetonic, chloroform, and n-hexane extracts.
  • Assessed free-radical scavenging (DPPH, FRAP, OH, NO), protection against H2O2-induced DNA damage, clot lysis, genotoxicity via comet assay, and cytotoxicity on HepG2 cells via MTT assay.
  • Conducted in silico molecular docking of 14 phytocompounds against target protein 51X0.
  • TPC and TFC were 51 ± 0.7 mg GAE/g and 66.5 ± 0.4 mg QE/g, respectively, with methanolic extracts demonstrating the strongest radical scavenging and a 33% clot lysis rate.
  • Comet assay established safety at 0.5 mg/mL, whereas the MTT assay demonstrated 86% HepG2 cell viability at 100 μg/mL of methanolic extract.
  • Molecular docking identified heptacosane, apiol, and palmitic acid as strong binding partners for target protein 51X0.

Cite This Study

Siddique et al. (2022) studied this question.

synapsesocial.com/papers/6a97a559883af1d6c8a676eahttps://doi.org/10.1016/j.arabjc.2022.103984
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Evaluation of the Phytochemical Screening of Methanolic Seed Extracts of Tribulus terrestris: An In Vitro Application of Anti-cancer, Anti-oxidant, and Anti-microbial Activities2024 · 2 citations
  2. 2Bioactive Compounds, Antibacterial, Antioxidant, Anticancer, and Antidiabetic Potential of the Seed and Leaves of Tribulus terrestris2025
  3. 3Bioactive Compounds, Antibacterial, Antioxidant, Anticancer, and Antidiabetic Potential of the Seed and Leaves of Tribulus terrestris2025
  4. 4Evaluation of The Antimicrobial, Antioxidant, and Other Biological Activities of Tribulus terrestris Plants Collected From Different Countries2024
  5. 5Assessment of Antioxidant Capacity, Phytochemical Phenolic Composition, Cholinesterase and α-Glucosidase Inhibitory Activities, and Antimicrobial Potential of Tribulus terrestris2026