L- and T-type Ca2+ channels in canine cardiac Purkinje cells. Single-channel demonstration of L-type Ca2+ window current.
Key result
Patch-clamp analysis of canine cardiac Purkinje cells identified L- and T-type Ca2+ channels with conductances of 24.2 pS and 9.0 pS (with Ba2+), demonstrating L-type window current transitions.
Population
Isolated canine cardiac Purkinje cells
Design
Preclinical
Authors
Discussion
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Member takes
Implication
May inform Purkinje arrhythmia models; leaves open human translation from canine data.
Structured PICO
P
Population
Isolated canine cardiac Purkinje cells
E
Exposure
On-cell patch-clamp technique with 110 mM Ba2+ or 110 mM Ca2+ as charge carriers
O
Outcome
Single-channel characterization of L- and T-type Ca2+ currents, including conductance and window currentsurrogate
Demonstrates the presence of L- and T-type Ca2+ channels and L-type window currents at the single-channel level in canine cardiac Purkinje cells, providing insights into potential mechanisms of arrhythmogenesis.
Cite This Study
Shorofsky et al. (1992) studied this question. Patch-clamp technique was evaluated on Single-channel conductance. Patch-clamp analysis of canine cardiac Purkinje cells identified L- and T-type Ca2+ channels with conductances of 24.2 pS and 9.0 pS (with Ba2+), demonstrating L-type window current transitions.