Key result
In vivo labelling of bovine enterovirus with 32P did not yield sufficient specific radioactivity for RNase T1 oligonucleotide mapping analysis.
Population
HeLa S3 cells infected with MI-320 strain of bovine enterovirus
Design
Preclinical
Authors
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Precludes in vivo 32P labelling for enterovirus mapping under limits; leaves open alternative methods for oligonucleotide analysis.
In vivo labelling of bovine enterovirus with H3 32PO4 did not yield sufficient specific radioactivity for RNase T1 oligonucleotide mapping analysis under the permitted radioactivity limits in Japan.
Hamada et al. (1985) studied Bovine enterovirus. 32P labelling (H3 32PO4) was evaluated on Specific radioactivity of virion RNA for RNase T1 oligonucleotide mapping. In vivo labelling of bovine enterovirus with 32P did not yield sufficient specific radioactivity for RNase T1 oligonucleotide mapping analysis.
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