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January 1, 1990Blood

Direct detection of activated platelets and platelet-derived microparticles in humans

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Authors

CACS AbramsUniversity of PennsylvaniaNENorig EllisonUniversity of PennsylvaniaABAZ BudzynskiTemple University

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Implication

Observational study reveals rapid platelet activation and microparticle release during hemostatic stress in humans, highlighting new diagnostic capabilities.

Key Points

  • To assess whether activated platelets and platelet-derived microparticles can be directly detected in human whole blood following hemostatic challenge using flow cytometry.
  • Evaluated two in vivo models of platelet activation: standardized bleeding time wounds in 9 normal subjects and 1 hour of cardiopulmonary bypass in 9 surgical patients.
  • Identified platelets and microparticles using biotinylated anti-GPIb with phycoerythrin-streptavidin, differentiating microparticles by light scatter.
  • Detected platelet activation using fluorescein-labeled monoclonal antibodies: PAC1 (activated GPIIb-IIIa), 9F9 (bound fibrinogen), and S12 (alpha-granule membrane protein).
  • In normal subjects, platelet activation markers PAC1, 9F9, and S12 increased progressively and were detected as early as 30 seconds after incision (P < .03), alongside decreasing wound platelet concentration and slight microparticle increases.
  • Cardiopulmonary bypass caused a 2.2-fold increase in the relative proportion of circulating microparticles (P < .001) and a significant 2- to 3-fold mean increase in PAC1 binding (P < .02).
  • PAC1 binding exceeded normal unstimulated ranges in 5 of 9 surgical patients, while 9F9 and S12 binding exceeded normal ranges in 2 of 9 patients.

Cite This Study

Abrams et al. (1990) studied this question.

synapsesocial.com/papers/6a9805e937fa270e2dfa93e2https://doi.org/10.1182/blood.v75.1.128.bloodjournal751128
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Also Consider

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