Key result
Reducing the net positive charge of loop 2 decreased myosin V's affinity for actin and processive run length, whereas adding positive charge increased both.
Population
Single molecules of myosin V and actin filaments
Comparison
Alteration of the net charge of loop 2 vs Myosin V with unaltered loop 2 charge / opposite…
Design
Preclinical
Authors
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Alters myosin V processivity in vitro; leaves open relevance to cellular transport and requires validation in physiological models.
The net charge of loop 2 in myosin V modulates its affinity for actin in the weak binding state, which directly determines its processive run length.
Hodges et al. (2007) studied this question. Altering the net charge of loop 2 was evaluated on Processive run length and affinity for actin. Reducing the net positive charge of loop 2 decreased myosin V's affinity for actin and processive run length, whereas adding positive charge increased both.
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