An outbreak of a novel recombinant Coxsackievirus A4 in a kindergarten, Shandong province, China, 2021

Figures

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  Figure 1.. (A) Phylogenetic analysis of 11 full-length CV-A4 genomes from the kindergarten outbreak and reference strains available from GenBank. Font colour in the phylogenetic tree represents the collection date of the CV-A4 strains. Maximum likelihood trees in this study were estimated using RAxML (version 8.1.6) [14] under the GTRGAMMA nucleotide substitution model with 1000 bootstrap replicates. The solid red circle represents the Chinese CV-A4 genomes identified by our laboratory, and the open red circle represents the Chinese CV-A4 genomes described by other laboratories. (B) Sequence identity between CV-A4 genomes from the kindergarten outbreak (exemplified by TA001K) and its most closely related strains. Similarity comparison of the potential recombination event in the TA001K genome was performed using Simplot [15], with a window size of 200 bp and a step size of 20 bp. The breakpoints were defined by RDP4 [16].