Key result
A newly established TaqMan real-time PCR assay detected Duck adeno-associated virus with a positive rate of 21.79% in 78 clinical samples from diseased or deceased ducklings.
Why the study?
Duck adeno-associated virus is a novel pathogen recently discovered in ducks, requiring a molecular detection assay for epidemiological investigation and pathogenic mechanism studies.
A newly developed TaqMan-qPCR assay provides a highly sensitive and specific method for detecting Duck adeno-associated virus, facilitating future epidemiological and pathogenic studies.
Provides sensitive DAAV detection tool in ducks; leaves open routine veterinary use pending prospective validation.
Duck adeno-associated Virus (DAAV) is a novel pathogen that was recently discovered in ducks. To establish a molecular detection assay for DAAV for further epidemiological investigation and pathogenic mechanism. Here, we designed specific primers and probes according to the sequence characteristics of the newly discovered DAAV and then established a TaqMan real-time PCR method (TaqMan-qPCR) for the detection of DAAV. Our data showed that the established TaqMan-qPCR for detecting DAAV had high sensitivity, with the lowest detection limit of 29.1 copies/μL. No cross reaction was found with duck circovirus (DuCV), H9N2 subtype avian influenza virus (AIV), avian Tembusu virus (ATmV). duck hepatitis A virus 1 and 3 (DHAV-1 and DHAV-3), duck adenovirus A (DAdV-A), duck adenovirus 3 (DAdV-3), or duck enteritis virus (DEV). The repeatability was excellent, with the coefficients of variation of repeated intragroup and intergroup tests ranging from 0.12-0.21% and 0.62-1.42%, respectively. Seventy-eight clinical samples collected from diseased or deceased ducklings were tested. The results showed that the DAAV positive rate was 21.79%, and a triple infection (DAAV+MDPV+GPV) was found. These data provide technical support for further molecular epidemiological surveillance and pathogenic mechanism studies of DAAV infection.
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Chen et al. (2024) studied Duck adeno-associated virus (DAAV) infection (n=78). TaqMan real-time PCR method (TaqMan-qPCR) for DAAV detection was evaluated on DAAV positive rate. A newly established TaqMan real-time PCR assay detected Duck adeno-associated virus with a positive rate of 21.79% in 78 clinical samples from diseased or deceased ducklings.
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