Key result
Vaccinia virus infection in HeLa S3 cells caused no dramatic redistribution of elements between nucleus and cytoplasm during the first 6 hours, though [Cl] increased relative to [Na] and [K].
X-ray microanalysis reveals specific elemental concentration changes, such as increased Cl relative to Na and K, in HeLa cells early after Vaccinia virus infection.
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These in vitro findings warrant no clinical application; they leave open whether early Cl/Na/K shifts influence vaccinia pathogenesis in vivo.
X-ray microanalysis has been used to study changes in concentration of P, S, Cl Na and K in synchronized HeLa S3 cells in g1 phase infected with Vaccinia virus. No dramatic redistribution of elements between nucleus and cytoplasm was demonstrated during the first 6 h post-infection, during which time viral shut-off of host protein synthesis occurred. [Cl] increased relative to [Na] and [K] during the first 3 h post-infection. The magnitude and direction of change in [K] and [Na] (from approximately 10 cells/analysis) was compared with data previously obtained by flame photometry (from approximately 10(6) cells/analysis) and found to be remarkably parallel.
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Warley et al. (1985) studied Synchronized HeLa S3 cells infected with Vaccinia virus. Vaccinia virus infection was evaluated on Changes in concentration of P, S, Cl, Na, and K. Vaccinia virus infection in HeLa S3 cells caused no dramatic redistribution of elements between nucleus and cytoplasm during the first 6 hours, though [Cl] increased relative to [Na] and [K].
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