Key result
Amiloride treatment significantly increased the mutation frequency of wild type Coxsackie virus B3 from 4.5 to 9.4 mutations per 10^4 nucleotides, revealing a previously unknown indirect mutagenic activity.
Population
Coxsackie virus B3 (CVB3) and poliovirus in HeLa cell cultures
Comparison
Amiloride compounds and RNA mutagens vs Untreated/mock-treated control cultures
Design
Preclinical
Authors
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Amiloride may have off-target mutagenic effects on RNA viruses; hypothesis-generating for antiviral strategies and should not change practice.
Absolute Event Rate: 9.4% vs 4.5%
p-value: p=0.0005
Amiloride compounds exhibit indirect mutagenic activity on RNA viruses by altering intracellular ion concentrations, which affects polymerase fidelity.
Levi et al. (2010) studied Coxsackie virus B3 (CVB3) and poliovirus infection (in vitro). Amiloride vs. Untreated control was evaluated on Mutation frequency of viral RNA genomes (mutations per 10^4 nucleotides) (p=0.0005). Amiloride treatment significantly increased the mutation frequency of wild type Coxsackie virus B3 from 4.5 to 9.4 mutations per 10^4 nucleotides, revealing a previously unknown indirect mutagenic activity.
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