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[9β‐3H]Prostaglandin F2α was incubated with the particle free fraction of a homogenate of guinea pig lung. One less polar metabolite appeared, which was assigned thje strcuture 9α.11α‐dihydroxy‐15‐ketoprost‐5‐enoic acid. The identification was based on gas chromatographic and mass spectrometric analyses of the trimethylsilyl ether, acetate, methoxime‐trimethlsily ether and methoxime‐acetate derivatives of the methyl ester. The position of the double bond was established by oxidative ozonolysis of the methlester and acetate followed by gas chromatogralphymass spectrometry.
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Elisabeth Granström (1971) studied this question.
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