Twelve Holstein cows were assigned to three blocks of a 4 4 Latin-square experimental design. The within-block treatments were: control, 1% Degras wool fat (48.3% cholesterol), 1% mixed soy sterols and a mixture of 0.5% Degras fat, and 0.5% of soy sterols of the concentrate mixture. The concentrates between blocks contained no added fat, 10% animal tallow, or 10% vegetable oil. Milk fat production, fat test, and fat-corrected milk production were highest when no sterol was added to the ration. Percentages solidsnot-fat, milk cholesterol, and blood fat were apparently not affected by the different sterols. However, cows. had lower blood cholesterol when a mixture of animal and plant sterols was fed compared to other groups.
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Adams et al. (1969) studied this question.