Key result
The IL-10 -1082 AA genotype was significantly more frequent in children with EV71 infection compared to healthy controls (59.9% vs 38.5%, p=0.001) and was associated with severe infection.
Why the study?
To evaluate the association of interleukin-10 gene polymorphisms with IL-10 levels and the severity of enterovirus 71 infection in children.
Case-Control (n=259)
Yes
Absolute Event Rate: 59.9% vs 38.5%
p-value: p=0.001
IL-10 gene polymorphisms, particularly the -1082 AA genotype, are associated with IL-10 expression levels and increased severity of enterovirus 71 infection in children.
IL-10 -1082 AA genotype associates with EV71 severity and lower IL-10 expression in children; leaves open whether genotyping informs risk stratification.
OBJECTIVE: To study the association of interleukin-10 (IL-10) -1082A/G, -819C/T, and -592C/A polymorphisms with IL-10 level and the severity of enterovirus 71 (EV71) infection in children. METHODS: A total of 137 children with hand-foot-mouth disease due to EV71 infection were enrolled as EV71 infection group, which was further divided into mild group with 91 children and severe group with 46 children, and 122 healthy children who underwent physical examination were enrolled as healthy control group. Related clinical data were collected. ELISA was used to measure the serum level of IL-10, and polymerase chain reaction-restriction fragment length polymorphism was used to analyze IL-10 -1082A/G, -819C/T and -592C/A polymorphisms. RESULTS: Compared with the healthy control group, the children with EV71 infection had significantly higher frequency of -1082 AA genotype and A allele (P<0.05). Among the children with EV71 infection, the severe group had significantly higher frequency of -1082 AA genotype and A allele than the mild group (P<0.05), while there was no significant difference in the distribution of IL-10 -819C/T and IL-10 -592C/A polymorphisms between the two groups (P>0.05). The severe group had a significantly higher serum level of IL-10 than the mild group and the healthy control group. IL-10 -1082 AA genotype, -819 TT genotype, and -592 AA genotype were associated with the low expression of IL-10 (P<0.05). As for haplotype, the EV71 infection group had a significantly lower frequency of GCC haplotype than the healthy control group (P<0.05). In the severe group, the children with ATA haplotype had a significantly lower IL-10 level than those with other haplotypes, and the children with GCC haplotype had a significantly higher IL-10 level than those with other haplotypes (P<0.05). There was no significant difference in IL-10 level between children with different haplotypes in the mild group and the healthy control group (P>0.05). CONCLUSIONS: IL-10 gene polymorphisms are associated with IL-10 expression and the severity of EV71 infection in children.
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Zhao et al. (2019) conducted a case-control in Enterovirus 71 (EV71) infection (hand-foot-mouth disease) (n=259). IL-10 gene polymorphisms (-1082A/G, -819C/T, -592C/A) vs. Healthy controls was evaluated on Frequency of IL-10 -1082 AA genotype (p=0.001). The IL-10 -1082 AA genotype was significantly more frequent in children with EV71 infection compared to healthy controls (59.9% vs 38.5%, p=0.001) and was associated with severe infection.
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