What question did this study set out to answer?

To determine the precise chromosomal localization of the protooncogene CBL2 on chromosome band 11q23 and assess its position relative to breakpoints involved in leukemia, lymphoma, and Ewing sarcoma.

May 13, 2008Cytogenetics and Cell Genetics

Relationship of the human protooncogene CBL2 on 11q23 to the t(4;11), t(11;22), and t(11;14) breakpoints

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Authors

PSPaul SavageMcGill University MSM. ShapiroCenter for Drug Evaluation and Research WLWallace Y. LangdonThe University of Western Australia

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Implication

Genetic mapping study demonstrates the location of protooncogene CBL2 relative to 11q23 translocation breakpoints, suggesting potential involvement in acute leukemia and lymphoma pathogenesis.

Key Points

To determine the precise chromosomal localization of the protooncogene CBL2 on chromosome band 11q23 and assess its position relative to breakpoints involved in leukemia, lymphoma, and Ewing sarcoma.
Hybridized a molecular probe for human CBL2 (homologous to murine v-cbl and Cbl-2) against panels of rodent-human somatic cell hybrids.
Mapped the gene relative to specific translocations using cell lines derived from acute leukemia with t(4;11)(q21;q23), B-cell lymphoma with t(11;14)(q23;q32), and Ewing sarcoma with t(11;22)(q23;q12).
CBL2 localized to 11q23, translocating to chromosome 4 in t(4;11) acute leukemia and chromosome 14 in t(11;14) B-cell lymphoma, while remaining on chromosome 11 in t(11;22) Ewing sarcoma.
Refined the regional gene and breakpoint order as centromere--NCAM--CD3(E-D-G)--[t(11;14), t(4;11)]--(THY1, CBL2, ETS1)--t(11;22)--telomere, with gross CBL2 structure remaining intact across flanking breakpoints.

Cite This Study

Savage et al. (2008) studied this question.

synapsesocial.com/papers/6a9aa36d141376a341869299 https://doi.org/10.1159/000133062

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Implication

Genetic mapping study demonstrates the location of protooncogene CBL2 relative to 11q23 translocation breakpoints, suggesting potential involvement in acute leukemia and lymphoma pathogenesis.

Relationship of the human protooncogene CBL2 on 11q23 to the t(4;11), t(11;22), and t(11;14) breakpoints

Discussion

Member takes

Implication

Key Points

Cite This Study

Relationship of the human protooncogene CBL2 on 11q23 to the t(4;11), t(11;22), and t(11;14) breakpoints

Discussion

Member takes

Implication

Key Points

Cite This Study

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