Key result
High performance liquid chromatography analysers achieved between-batch coefficients of variation less than 5%, demonstrating better precision than low pressure chromatography and immunoassay systems.
High performance liquid chromatography provides superior precision for glycated haemoglobin measurement compared to low pressure chromatography and immunoassay systems.
May support HPLC preference for HbA1c testing in labs; leaves open effects on clinical decisions or outcomes.
We have assessed five high-throughput systems for the measurement of glycated haemoglobin and have reviewed published evaluations of individual analysers. All systems offered better precision than a widely used electroendosmosis method. The low pressure chromatography and immunoassay systems demonstrated greater between-batch imprecision than the high performance liquid chromatography analysers, the latter achieving the proposed analytical goal of between-batch coefficients of variation less than 5%. Agreement between all systems measuring HbA1 was good but there was variability amongst observed HbA1c values. The systems were also assessed for their quality of chromatographic separation, simplicity of operation, flexibility, cost and potential for interference by other haemoglobins.
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Standing et al. (1992) studied this question. High-throughput systems for glycated haemoglobin measurement vs. Electroendosmosis method was evaluated on Precision (between-batch coefficients of variation). High performance liquid chromatography analysers achieved between-batch coefficients of variation less than 5%, demonstrating better precision than low pressure chromatography and immunoassay systems.
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