Key result
Both the immunomagnetic separation real-time RT-PCR and the reporter cell assay detected infective enteroviruses in environmental water samples at the same level as the conventional plaque assay.
Effect estimate: R2 > 0.9
p-value: p=0.3982
IMS RT-PCR and a FRET-based reporter assay perform as well as the conventional plaque assay for detecting infectious enteroviruses in environmental water samples.
Matches plaque assay for enterovirus detection in water; extends rapid options but leaves open routine surveillance adoption.
Two newly developed protocols for infective virus detection were compared to the plaque assay. An immunomagnetic separation procedure coupled with real-time reverse transcription-PCR of viral nucleic acids was developed to identify intact enteroviral particles, and a reporter cell system responding to viral replication based on fluorescent resonance energy transfer for detection of infectious enteroviruses was tested. Both new procedures detected infective viruses in environmental samples at the same level as the plaque assay.
No takes yet. Share an insight, caveat, or question.
Hwang et al. (2007) studied Enterovirus detection in environmental water. Immunomagnetic separation real-time RT-PCR and FRET-based reporter assay vs. Plaque assay was evaluated on Detection of infective enteroviruses (correlation with plaque assay) (R2 > 0.9, p=0.3982). Both the immunomagnetic separation real-time RT-PCR and the reporter cell assay detected infective enteroviruses in environmental water samples at the same level as the conventional plaque assay.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: