Key result
A quartz crystal microbalance sensor using molecularly imprinted polymer detected classical swine fever virus with a limit of detection of 1.7 μg/mL and high selectivity over other porcine viruses.
Why the study?
Classical swine fever is a highly contagious and fatal viral disease in pigs, dictating economic need for rapid early detection of the pathogen.
A novel QCM-based sensor using molecularly imprinted polymers enables rapid, selective, and reversible detection of classical swine fever virus.
Classical swine fever (CSF) is a highly contagious and fatal viral disease in pigs caused by the virus of the same name (classical swine fever virus – CSFV). Economical reasons dictate the need for rapid early detection of this pathogen. Herein we report on a sensor for CSFV detection based on a quartz crystal microbalance (QCM) making use of molecularly imprinted polymer (MIP) as the receptor. It relies on a copolymer comprising acrylamide (AAM), methacrylic acid (MAA), methyl methacrylate (MMA), and n-vinylpyrrolidone (VP). SEM images of CSFV MIP reveal cavities on the polymer surface with an average diameter of d=59 nm, which correlates well with the dimensions of CSFV particles. QCM sensor measurements yield concentration-dependent CSFV sensor responses resulting in LOD=1.7 μg/mL, LOQ=5.1 μg/mL and R 2 = 0.9963. Furthermore, CSFV-MIP sensors selectively bind CSFV with selectivity factors of 2 over porcine respiratory and reproductive virus (PRRSV) and 62 over pseudorabies virus (PRV), respectively. Finally, sensor responses turned out fully reversible.
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Klangprapan et al. (2020) studied Classical swine fever (CSF). Quartz crystal microbalance (QCM) sensor with molecularly imprinted polymer (MIP) was evaluated on Limit of detection (LOD) and limit of quantification (LOQ) for CSFV. A quartz crystal microbalance sensor using molecularly imprinted polymer detected classical swine fever virus with a limit of detection of 1.7 μg/mL and high selectivity over other porcine viruses.
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