A magnetoelastic biosensor based on E2 glycoprotein for wireless detection of classical swine fever virus E2 antibody

Figures

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  Figure 1. Schematic representation of the procedures of the ME biosensors functionalization.

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  Figure 2. The schematic representation of wireless ME biosensor measurement system.

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  Figure 3. (a) SEM image of the gold-coated sensor surface. (b) SEM image of biosensor surface after CSFV E2 immobilization. (c) EDS spectrum of the biosensor surface before and after the CSFV E2 immobilization.

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  Figure 4. (a) Schematic diagram of FITC-labeled anti-CSFV E2 antibody captured by CSFV E2 modified on the biosensor surface. Fluorescence images of biosensors coated with CSFV E2 at different concentrations, including (b) 0 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\rm{\mu }}{\rm{g}}/{\rm{mL}}$$\end{document}μg/mL, (c) 10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\rm{\mu }}{\rm{g}}/{\rm{mL}}$$\end{document}μg/mL, (d) 30 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\rm{\mu }}{\rm{g}}/{\rm{mL}}$$\end{document}μg/mL, (e) 50 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb}…

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  Figure 5. Time-dependent frequency responses at different anti-CSFV E2 antibody concentrations ranging from 0 to 10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\rm{\mu }}{\rm{g}}/{\rm{mL}}$$\end{document}μg/mL.

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  Figure 6. Calibration curve: the 50 min shift in resonance frequency as a function of different anti-CSFV E2 antibody concentrations.

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  Figure 7. Real-time response of the ME biosensor to other interferents with the concentration of 10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\rm{ng}}/{\rm{mL}}$$\end{document}ng/mL.