Key result
Mechanical stress from rolling and crimping up to 10 minutes did not significantly affect the stability of colonized cells on polyurethane patches, demonstrating the feasibility of tissue-engineered transcatheter heart valves.
Why the study?
Does mechanical stress from rolling and crimping affect the cellular coating integrity of tissue-engineered polyurethane scaffolds?
Does mechanical stress from rolling and crimping affect the cellular coating integrity of tissue-engineered polyurethane scaffolds?
Tissue-engineered polyurethane scaffolds seeded with human vascular cells maintain cellular integrity after rolling and crimping, supporting their potential use in transcatheter heart valves.
Cellular stability after crimping supports further preclinical testing of tissue-engineered valves; leaves open in vivo durability and clinical translation.
Transcatheter valve replacement using bioprosthetics is a common procedure, however limited due to graft durability. The aim of this study was to verify the mechanical durability of tissue engineered constructs to crimping necessary for transcatheter deployment. Polyurethane patches were seeded with human vascular cells. Mechanical stress of transcatheter methods was simulated by rolling and crimping. Seeded patches were rolled using a patch-rolling device. For crimping, seeded patches were fixed on a polymer stent and crimped by a conventional crimper. Effect of mechanical strain on cellular coating was analyzed by scanning electron microscopy (SEM), immunocytochemistry (ICC) and immunohistochemistry (IHC). SEM analysis of stressed patches showed a confluent cellular coating. ICC and IHC demonstrated the integrity of cellular layers after rolling and crimping. Mechanical stress does not affect the stability of colonized cells on polyurethane patches. This demonstrates the feasibility of applying tissue-engineering methods for the development of seeded transcatheter heart valves.
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Scheuer et al. (2012) studied Tissue engineering for transcatheter heart valves (n=88). Mechanical stress (rolling and crimping) vs. Seeded patches without mechanical stress was evaluated on Mechanical durability and integrity of cellular coating. Mechanical stress from rolling and crimping up to 10 minutes did not significantly affect the stability of colonized cells on polyurethane patches, demonstrating the feasibility of tissue-engineered transcatheter heart valves.
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